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タイトル: Isolation of an oxalate-resistant Ashbya gossypii strain and its improved riboflavin production.
著者: Sugimoto, Takashi
Morimoto, Aki
Nariyama, Masashi
Kato, Tatsuya
Park, Enoch Y.
掲載誌名: Journal of industrial microbiology & biotechnology
出版者: Springer
巻: 37
号: 1
開始ページ: 57
終了ページ: 64
出版日付: 2010-01
権利: The original publication is available at
NDC: 474
抄録: An oxalate-resistant strain of Ashbya gossypii was naturally isolated from spores grown on an oxalate-containing medium, and its medium was optimized to improve riboflavin production. Riboflavin production by the resistant strain was three-fold higher than that by the wild-type organism when grown in flask cultures. Medium optimization increased the riboflavin production by the resistant strain to 5 g l(-1), which was five-fold higher than that obtained by the wild-type strain. The productivity was reproduced in a 3-l bioreactor. During the early growth phase, the specific activity of isocitrate lyase in the oxalate-resistant strain was slightly higher than that in the wild-type strain. Proteomic analysis of the oxalate-resistant strain revealed that the expression of aldose reductase and cobalamin-independent methionine synthase decreased significantly. This is the first report that describes the natural isolation of a riboflavin producer using an antimetabolite-containing medium to enhance the riboflavin production level. This method should also be useful for improving the productivity of other bioproducts since it does not require any mutations or genetic modifications of the microorganism.
ISSN: 13675435 OPAC
E-ISSN: 14765535 OPAC
出版者DOI: 10.1007/s10295-009-0647-3   
PMID: 19826846
NII書誌ID: AA11132053 OPAC ciniib
バージョン: publisher
出現コレクション:11. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Sugimoto, Takashi -- Morimoto, Aki -- Nariyama, Masashi -- Kato, Tatsuya (加藤, 竜也) (カトウ, タツヤ) -- Park, Enoch Y. (朴, 龍洙) (パク, ヨンス)



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