Shizuoka University REpository  title image 

SURE: Shizuoka University REpository >
11. 創造科学技術大学院 = Graduate School of Science and Technology >
11. 雑誌論文・記事(Journal Article, Article, Preprint) >

Bookmark and Share


ファイル 記述 サイズフォーマット
100702004.pdf675.38 kBAdobe PDFサムネイル

タイトル: Response of cellulase activity in pH-controlled cultures of the filamentous fungus Acremonium cellulolyticus.
著者: Prasetyo, Joni
Sumita, Shyuuhei
Okuda, Naoyuki
Park, Enoch Y.
掲載誌名: Applied Biochemistry and Biotechnology
出版者: Springer
巻: 162
号: 1
開始ページ: 52
終了ページ: 61
出版日付: 2010-09
権利: The original publication is available at
NDC: 464
抄録: Cellulase production was investigated in pH-controlled cultures of Acremonium cellulolyticus. The response to culture pH was investigated for three cellulolytic enzymes, carbomethyl cellulase (CMCase), avicelase, and beta-glucosidase. Avicelase and beta-glucosidase showed similar profiles, with maximum activity in cultures at pH 5.5-6. The CMCase activity was highest in a pH 4 culture. At an acidic pH, the ratios of CMCase and avicelase activity to cellulase activity defined by filter paper unit were high, but at a neutral pH, the beta-glucosidase ratio was high. The pH 6.0 culture showed the highest cellulase activity within the range of pH 3.5-6.5 cultures. The saccharification activity from A. cellulolyticus was compared to those of the cellulolytic enzymes from other species. The A. cellulolyticus culture broth had a saccharification yield comparable to those of the Trichoderma enzymes GC220 and Cellulosin T2, under conditions with the same cellulase activity. The saccharification yields from Solka floc, Avicel, and waste paper, measured as the percent of released reducing sugar to dried substrate, were greater than 80% after 96 h of reaction. The yields were 16% from carboxymethylcellulose and 26% from wood chip refiner. Thus, the A. cellulolyticus enzymes were suitable for converting cellulolytic biomass to reducing sugars for biomass ethanol production. This study is a step toward the establishment of an efficient system to reutilize cellulolytic biomass.
ISSN: 02732289 OPAC
E-ISSN: 15590291 OPAC
出版者DOI: 10.1007/s12010-009-8826-2   
PMID: 19882113
NII書誌ID: AA10621790 OPAC ciniib
バージョン: publisher
出現コレクション:11. 雑誌論文・記事(Journal Article, Article, Preprint)

Google™ Scholar: Cited By - Related - Other Copies

Others By: Prasetyo, Joni -- Sumita, Shyuuhei -- Okuda, Naoyuki -- Park, Enoch Y. (朴, 龍洙) (パク, ヨンス)



Powered by DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - ご意見をお寄せください