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タイトル: Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure
著者: Park, Enoch Y.
Ishikiriyama, Motoki
Nishina, Takuya
Kato, Tatsuya
Yagi, Hirokazu
Kato, Koichi
Ueda, Hiroshi
掲載誌名: Journal of Biotechnology
出版者: Elsevier
巻: 139
号: 1
開始ページ: 108
終了ページ: 114
出版日付: 2009-01-01
権利: Copyright © 2008 Elsevier B.V. All rights reserved.
NDC: 465
抄録: A Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains of human 29IJ6 IgG was constructed and used to secrete recombinant antibody into silkworm larval hemolymph. Fifth instar silkworm larvae were reared and injected into the dorsum of the larvae with recombinant cysteine protease- and chitinase-deficient BmNPV (BmNPV-CP(-)-Chi(-)) bacmid/29IJ6 IgG and harvested after approximately 6 days. The total yield of recombinant 29IJ6 IgG was 36 microg/larvae, which is equivalent to 8 mg/kg of larvae. The recombinant antibody was purified to homogeneity using a HiTrap rProtein A FF column with a purification yield of 83.1%. The purified protein was identified by Western blot and ELISA experiments. The N-linked glycan structure of the purified protein was determined by the HPLC mapping method. The N-glycans of the 29IJ6 IgG glycoprotein produced in, and secreted by the silkworm larvae were composed exclusively of two kinds of paucimannose-type oligosaccharides, Manalpha1-6Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc and Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc.
ISSN: 01681656 OPAC
出版者DOI: 10.1016/j.jbiotec.2008.09.013   
PMID: 18984019
NII書誌ID: AA10458361 OPAC ciniib
バージョン: author
出現コレクション:11. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Park, Enoch Y. (朴, 龍洙) (パク, ヨンス) -- Ishikiriyama, Motoki -- Nishina, Takuya -- Kato, Tatsuya (加藤, 竜也) (カトウ, タツヤ) -- Yagi, Hirokazu -- Kato, Koichi -- Ueda, Hiroshi



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