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タイトル: Characterization of plasma triiodophenol binding proteins in vertebrates and tissue distribution of triiodophenol in Rana catesbeiana tadpoles
著者: Yamauchi, Kiyoshi
Sai, Gobun
掲載誌名: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
出版者: Elsevier
巻: 153
号: 3
開始ページ: 328
終了ページ: 335
出版日付: 2011-04
権利: Copyright © 2010 Elsevier Inc. All rights reserved.
NDC: 464
抄録: We investigated the interaction of 2,4,6-triiodophenol (TIP), a potent thyroid hormone disrupting chemical, with serum proteins from rainbow trout (Onchorhynchus mykiss), bullfrog (Rana catesbeiana), chicken (Gallus gallus), pig (Sus scrofa domesticus), and rat (Rattus norvegicus) using a [(125)I]TIP binding assay, gel filtration chromatography, and native polyacrylamide gel electrophoresis. [(125)I]TIP bound non-specifically to proteins in trout serum, specifically but weakly to proteins in bullfrog serum, and specifically and strongly to proteins in chicken, pig, and rat serum samples. Candidate TIP-binding proteins included lipoproteins (220-320kDa) in trout, albumin in bullfrog, albumin and transthyretin (TTR) in chicken and pig, and TTR in rat. TTR in the chicken, pig, and rat serum samples was responsible for the high-affinity, low-capacity binding sites for TIP (dissociation constant 2.2-3.5×10(-10)M). In contrast, a weak interaction of [(125)I]TIP with tadpole serum proteins accelerated [(125)I]TIP cellular uptake in vitro. Intraperitoneal injection of [(125)I]TIP in tadpoles revealed that the radioactivity was predominantly accumulated in the gallbladder and the kidney. The differences in the molecular and binding properties of TIP binding proteins among vertebrates would affect in part the cellular availability, tissue distribution and clearance of TIP.
ISSN: 15320456 OPAC
出版者DOI: 10.1016/j.cbpc.2010.12.003   
PMID: 21147258
NII書誌ID: AA11504679 OPAC ciniib
バージョン: author
出現コレクション:04. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Yamauchi, Kiyoshi (山内, 清志) (ヤマウチ, キヨシ) -- Sai, Gobun



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