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タイトル: Apoptosis at Inflection Point in Liquid Culture of Budding Yeasts
著者: Hagiwara, Toshiyuki
Ushimaru, Takashi
Tainaka, Kei-ichi
Kurachi, Hironori
Yoshimura, Jin
掲載誌名: PLoS ONE
出版者: Public Library of Science
巻: 6
号: 4
開始ページ: e19224
出版日付: 2011-04-27
権利: © 2011 Hagiwara et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
NDC: 461
抄録: Budding yeasts are highly suitable for aging studies, because the number of bud scars (stage) proportionally correlates with age. Its maximum stages are known to reach at 20–30 stages on an isolated agar medium. However, their stage dynamics in a liquid culture is virtually unknown. We investigate the population dynamics by counting scars in each cell. Here one cell division produces one new cell and one bud scar. This simple rule leads to a conservation law: ‘‘The total number of bud scars is equal to the total number of cells.’’ We find a large discrepancy: extremely fewer cells with over 5 scars than expected. Almost all cells with 6 or more scars disappear within a short period of time in the late log phase (corresponds to the inflection point). This discrepancy is confirmed directly by the microscopic observations of broken cells. This finding implies apoptosis in older cells (6 scars or more).
E-ISSN: 19326203 OPAC
出版者DOI: 10.1371/journal.pone.0019224   
PMID: 21556367
バージョン: publisher
出現コレクション:11. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Hagiwara, Toshiyuki -- Ushimaru, Takashi (丑丸, 敬史) (ウシマル, タカシ) -- Tainaka, Kei-ichi (泰中, 啓一) (タイナカ, ケイイチ) (Tainaka, Keiichi) -- Kurachi, Hironori -- Yoshimura, Jin (吉村, 仁) (ヨシムラ, ジン)



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