Shizuoka University
Library
|
SURE: Shizuoka University REpository >
06. 農学部・農学研究科 = Faculty of Agriculture >
06. 雑誌論文・記事(Journal Article, Article, Preprint) >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10297/5639
|
| Title: | Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete sordida YK-624 |
| Authors: | Sugiura, Tatsuki
Yamagishi, Kenji
Kimura, Toshiyuki
Nishida, Tomoaki
Kawagishi, Hirokazu
Hirai, Hirofumi |
| Journal Title: | Bioscience, Biotechnology, and Biochemistry |
| Publisher: | Japan Society for Bioscience, Biotechnology, and Agrochemistry |
| Journal Volume: | 73 |
| Journal Issue: | 8 |
| Start Page: | 1793 |
| End Page: | 1798 |
| Issue Date: | 2009 |
| NDC: | 658 |
| Abstract: | Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-LiP1 respectively. The promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from P. sordida YK-624 (PsGPD) was used to drive the expression of ylpA. Expression vector pGPD-g-ylpA was transformed into a P. sordida YK-624 uracil auxotrophic mutant, UV-64. The YlpA protein was secreted in active form by the transformants after 4 d of growth in a medium containing an excessive nitrogen source, whereas endogenous YK-LiP1 and YK-LiP2 were not produced. The physical and catalytic properties of the purified YlpA protein were very similar to those of YK-LiP2. These results suggest that homologous expression of recombinant YK-LiP2 was successful. |
| ISSN: | 09168451  |
| E-ISSN: | 13476947 |
| DOI: | 10.1271/bbb.90152 |
| PMID: | 19661691 |
| Article NAID: | 130000119582  |
| Journal NCID: | AA10824164  |
| Textversion: | publisher |
| Appears in Collections: | 06. 雑誌論文・記事(Journal Article, Article, Preprint)
|
Google™ Scholar:
Cited By -
Related -
Other Copies
Others By:
Sugiura, Tatsuki
-- Yamagishi, Kenji
-- Kimura, Toshiyuki
-- Nishida, Tomoaki
( 西田, 友昭)
( ニシダ, トモアキ)
-- Kawagishi, Hirokazu
( 河岸, 洋和)
( カワギシ, ヒロカズ)
-- Hirai, Hirofumi
( 平井, 浩文)
( ヒライ, ヒロフミ)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
|
){kind=link}
){kind=link}
){kind=link}
){kind=link}