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Title: Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete sordida YK-624
Authors: Sugiura, Tatsuki
Yamagishi, Kenji
Kimura, Toshiyuki
Nishida, Tomoaki
Kawagishi, Hirokazu
Hirai, Hirofumi
Journal Title: Bioscience, Biotechnology, and Biochemistry
Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Journal Volume: 73
Journal Issue: 8
Start Page: 1793
End Page: 1798
Issue Date: 2009
NDC: 658
Abstract: Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-LiP1 respectively. The promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from P. sordida YK-624 (PsGPD) was used to drive the expression of ylpA. Expression vector pGPD-g-ylpA was transformed into a P. sordida YK-624 uracil auxotrophic mutant, UV-64. The YlpA protein was secreted in active form by the transformants after 4 d of growth in a medium containing an excessive nitrogen source, whereas endogenous YK-LiP1 and YK-LiP2 were not produced. The physical and catalytic properties of the purified YlpA protein were very similar to those of YK-LiP2. These results suggest that homologous expression of recombinant YK-LiP2 was successful.
ISSN: 09168451 OPAC
E-ISSN: 13476947 OPAC
DOI: 10.1271/bbb.90152   
PMID: 19661691
Article NAID: 130000119582 ciniia
Journal NCID: AA10824164 OPAC ciniib
Textversion: publisher
Appears in Collections:06. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Sugiura, Tatsuki -- Yamagishi, Kenji -- Kimura, Toshiyuki -- Nishida, Tomoaki (西田, 友昭) (ニシダ, トモアキ) -- Kawagishi, Hirokazu (河岸, 洋和) (カワギシ, ヒロカズ) -- Hirai, Hirofumi (平井, 浩文) (ヒライ, ヒロフミ)

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