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タイトル: Functional characterization of rose phenylacetaldehyde reductase (PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol
著者: Chen, Xiao-Min
Kobayashi, Hiromi
Sakai, Miwa
Hirata, Hiroshi
Asai, Tatsuo
Ohnishi, Toshiyuki
Susanne, Baldermann
Watanabe, Naoharu
掲載誌名: Journal of Plant Physiology
出版者: Elsevier
巻: 168
号: 2
開始ページ: 88
終了ページ: 95
出版日付: 2011-01-15
権利: Copyright © 2010 Elsevier GmbH All rights reserved.
NDC: 471
抄録: 2-Phenylethanol (2PE) is a prominent scent compound released from flowers of Damask roses (Rosa×damascena) and some hybrid roses (Rosa 'Hoh-Jun' and Rosa 'Yves Piaget'). 2PE is biosynthesized from l-phenylalanine (l-Phe) via the intermediate phenylacetaldehyde (PAld) by two key enzymes, aromatic amino acid decarboxylase (AADC) and phenylacetaldehyde reductase (PAR). Here we describe substrate specificity and cofactor preference in addition to molecular characterization of rose-PAR and recombinant PAR from R.×damascena. The deduced amino acid sequence of the full-length cDNA encoded a protein exhibiting 77% and 75% identity with Solanum lycopersicum PAR1 and 2, respectively. The transcripts of PAR were higher in petals than calyxes and leaves and peaking at the unfurling stage 4. Recombinant PAR and rose-PAR catalyzed reduction of PAld to 2PE using NADPH as the preferred cofactor. Reductase activity of rose-PAR and recombinant PAR were higher for aromatic and aliphatic aldehydes than for keto-carbonyl groups. Both PARs showed that (S)-[4-(2)H] NADPH was preferentially used over the (R)-[4-(2)H] isomer to give [1-(2)H]-2PE from PAld, indicating that PAR can be classified as short-chain dehydrogenase reductase (SDR).
ISSN: 01761617 OPAC
出版者DOI: 10.1016/j.jplph.2010.06.011   
PMID: 20650544
NII書誌ID: AA10522948 OPAC ciniib
バージョン: author
出現コレクション:11. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Chen, Xiao-Min (陳, 暁敏) (チェン, ションミン) (Chen, Xiao-min) -- Kobayashi, Hiromi -- Sakai, Miwa -- Hirata, Hiroshi -- Asai, Tatsuo (浅井, 辰夫) (アサイ, タツオ) -- Ohnishi, Toshiyuki (大西, 利幸) (オオニシ, トシユキ) -- Susanne, Baldermann (スザンヌ, バルダーマン) (スザンヌ, バルダーマン) -- Watanabe, Naoharu (渡邉, 修治) (ワタナベ, ナオハル)



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