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タイトル: Expression of an RSV-gag virus-like particle in insect cell lines and silkworm larvae
著者: Deo, Vipin Kumar
Tsuji, Yoshitaka
Yasuda, Tomomi
Kato, Tatsuya
Sakamoto, Naonori
Suzuki, Hisao
Park, Enoch Y.
掲載誌名: Journal of Virological Methods
出版者: Elsevier
巻: 177
号: 2
開始ページ: 147
終了ページ: 152
出版日付: 2011-11
権利: Copyright © 2011 Published by Elsevier B.V.
NDC: 464
抄録: Rous sarcoma virus group antigen protein-based virus-like particles (VLPs) are well known for their structural integrity and ease of handling. VLPs play an important role in drug delivery systems because they can be manipulated with ease. In this study, a new method was established for expressing Rous sarcoma virus group antigen protein based VLPs in silkworm larvae and establishing stably expressing insect cell lines. These VLPs have been isolated by ultracentrifugation using a sucrose step gradient of 10-60% (v/v), and their spherical structure has been confirmed using transmission electron microscopy (TEM). The spherical morphology is similar in both the silkworm larvae and in stably expressing cell lines. Silkworm larvae are better suited for producing Rous sarcoma virus group antigen protein-based VLPs on a large scale; yields from silkworm larvae were approximately 8.2-fold higher than yields from stable cell lines. These VLPs provide a new method for large-scale application in vaccine development and drug delivery systems.
ISSN: 01660934 OPAC
E-ISSN: 18790984 OPAC
出版者DOI: 10.1016/j.jviromet.2011.07.012   
PMID: 21816175
NII書誌ID: AA00259584 OPAC ciniib
バージョン: author
出現コレクション:11. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Deo, Vipin Kumar (デオ, ヴィピン クマル) (デオ, ヴィピン クマル) -- Tsuji, Yoshitaka -- Yasuda, Tomomi -- Kato, Tatsuya (加藤, 竜也) (カトウ, タツヤ) -- Sakamoto, Naonori (坂元, 尚紀) (サカモト, ナオノリ) -- Suzuki, Hisao (鈴木, 久男) (スズキ, ヒサオ) -- Park, Enoch Y. (朴, 龍洙) (パク, ヨンス)



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