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タイトル: The improvement of riboflavin production in Ashbya gossypii via disparity mutagenesis and DNA microarray analysis
著者: Park, Enoch Y.
Ito, Yoko
Nariyama, Masashi
Sugimoto, Takashi
Lies, Dwiarti
Kato, Tatsuya
掲載誌名: Applied Microbiology and Biotechnology
出版者: Springer
巻: 91
号: 5
開始ページ: 1315
終了ページ: 1326
出版日付: 2011-09
権利: Copyright © 2011, Springer-Verlag
NDC: 464
抄録: We generated a high riboflavin-producing mutant strain of Ashbya gossypii by disparity mutagenesis using mutation of DNA polymerase δ in the lagging strand, resulting in loss of DNA repair function by the polymerase. Among 1,353 colonies generated in the first screen, 26 mutants produced more than 3 g/L of riboflavin. By the second screen and single-colony isolation, nine strains that produced more than 5.2 g/L of riboflavin were selected as high riboflavin-producing strains. These mutants were resistant to oxalic acid and hydrogen peroxide as antimetabolites. One strain (W122032) produced 13.7 g/L of riboflavin in a 3-L fermentor using an optimized medium. This represents a ninefold improvement on the production of the wild-type strain. Proteomic analysis revealed that ADE1, RIB1, and RIB5 proteins were expressed at twofold higher levels in this strain than in the wild type. DNA microarray analysis showed that purine and riboflavin biosynthetic pathways were upregulated, while pathways related to carbon source assimilation, energy generation, and glycolysis were downregulated. Genes in the riboflavin biosynthetic pathway were significantly overexpressed during both riboflavin production and stationary phases, for example, RIB1 and RIB3 were expressed at greater than sixfold higher levels in this strain compared to the wild type. These results indicate that the improved riboflavin production in this strain is related to a shift in carbon flux from β-oxidation to the riboflavin biosynthetic pathway.
ISSN: 01757598 OPAC
E-ISSN: 14320614 OPAC
出版者DOI: 10.1007/s00253-011-3325-0   
PMID: 21573938
NII書誌ID: AA10503386 OPAC ciniib
バージョン: author
出現コレクション:11. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Park, Enoch Y. (朴, 龍洙) (パク, ヨンス) -- Ito, Yoko -- Nariyama, Masashi -- Sugimoto, Takashi -- Lies, Dwiarti -- Kato, Tatsuya (加藤, 竜也) (カトウ, タツヤ)



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