Shizuoka University REpository  title image 

SURE: Shizuoka University REpository >
06. 農学部・農学研究科 = Faculty of Agriculture >
06. 雑誌論文・記事(Journal Article, Article, Preprint) >

Bookmark and Share


ファイル 記述 サイズフォーマット
120410002.pdf185.96 kBAdobe PDFサムネイル

タイトル: Sperm acrosin is responsible for the sperm binding to the egg envelope during fertilization in Japanese quail (Coturnix japonica)
著者: Sasanami, Tomohiro
Yoshizaki, Norio
Dohra, Hideo
Kubo, Hideo
掲載誌名: Reproduction
出版者: Society for Reproduction and Fertility
巻: 142
号: 2
開始ページ: 267
終了ページ: 276
出版日付: 2011-06-03
権利: © 2011 Society for Reproduction and Fertility
NDC: 464
抄録: An antibody library against quail sperm plasma membrane components was established and a mAb, which strongly inhibits sperm perforations of the perivitelline membrane (PVM) was obtained from the library. The antigen molecule of the mAb showed an apparent molecular weight of 45  kDa, and was distributed both on the surface and in the acrosomal matrix of the sperm head. Periodate oxidation revealed that the epitope of the antigen includes a sugar moiety. Tandem mass spectrometry analysis of the antigen revealed that the mAb recognizes sperm acrosin. When sodium dodecyl sulfate-solubilized PVM immobilized on a polyvinylidene difluoride membrane was incubated with sperm plasma membrane lysates, the sperm acrosin was detected on the PVM immobilized on the membrane, indicating that the sperm acrosin interacts with the components of PVM. Indeed, the mAb effectively inhibited the binding of acrosome-intact sperm to the PVM. These results indicate that the 45  kDa sperm acrosin is involved in the binding of sperm to the PVM in fertilization of Japanese quail.
ISSN: 14701626 OPAC
E-ISSN: 17417899 OPAC
出版者DOI: 10.1530/REP-11-0120   
PMID: 21642383
NII書誌ID: AA11501251 OPAC ciniib
バージョン: author
出現コレクション:06. 雑誌論文・記事(Journal Article, Article, Preprint)

Google™ Scholar: Cited By - Related - Other Copies

Others By: Sasanami, Tomohiro (笹浪, 知宏) (ササナミ, トモヒロ) -- Yoshizaki, Norio -- Dohra, Hideo (道羅, 英夫) (ドウラ, ヒデオ) -- Kubo, Hideo



Powered by DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - ご意見をお寄せください