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タイトル: Progesterone Is a Sperm-Releasing Factor from the Sperm-Storage Tubules in Birds
著者: Ito, Tomomi
Yoshizaki, Norio
Tokumoto, Toshinobu
Ono, Hiroko
Yoshimura, Takashi
Tsukada, Akira
Kansaku, Norio
Sasanami, Tomohiro
掲載誌名: Endocrinology
出版者: Endocrine Society
巻: 152
号: 10
開始ページ: 3952
終了ページ: 3962
出版日付: 2011-08-02
権利: Copyright © 2011 by The Endocrine Society
NDC: 464
抄録: Because of the presence of sperm-storage tubules (SST) in the utero-vaginal junction (UVJ) in the oviduct, once ejaculated sperm have entered the female reproductive tract, they can survive for a prolonged time in domestic birds, although the specific mechanisms involved in the sperm uptake into, maintenance within, and controlled release from the SST remain to be elucidated. In this report, we provide evidence that progesterone triggers the release of the resident sperm from the SST in the UVJ. The ultrastructural observation of the SST indicated that the resident sperm are released from the SST around 20 h after oviposition. When laying birds were injected with progesterone, most of the sperm were released from the SST within 1 h of injection. In situ hybridization analyses demonstrated the presence of the transcripts of membrane progestin receptor α in the UVJ, and the translated proteins were detected in the UVJ extracts by Western blotting. Moreover, the number of secretory granules in the SST epithelial cells fluctuates during the ovulatory cycle, and the progesterone administration mimics this phenomena. A binding assay using [(3)H]-progesterone indicated the presence of a high affinity, limited capacity, saturable and single binding site for [(3)H]-progesterone in the membrane fraction of the UVJ, and this receptor did not interact with the synthetic antiprogestin RU486. These results demonstrated for the first time that the progesterone stimulates the release of the resident sperm from the SST and that the release of the sperm might occur via membrane progestin receptor α-mediating signal transduction.
E-ISSN: 19457170 OPAC
出版者DOI: 10.1210/en.2011-0237   
PMID: 21810949
NII書誌ID: AA12024486 OPAC ciniib
バージョン: author
出現コレクション:06. 雑誌論文・記事(Journal Article, Article, Preprint)

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Others By: Ito, Tomomi -- Yoshizaki, Norio -- Tokumoto, Toshinobu (徳元, 俊伸) (トクモト, トシノブ) -- Ono, Hiroko -- Yoshimura, Takashi -- Tsukada, Akira -- Kansaku, Norio -- Sasanami, Tomohiro (笹浪, 知宏) (ササナミ, トモヒロ)



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